The inciting incident or what gets this particular story going
Peter's conflict:
Peter is changing and he’s angry that his Uncle Ben is giving him a lecture on responsibility. They’re not talking about the same thing, because Uncle Ben doesn’t know about the bite. But Peter does—and he’s fighting his subconscious motivation (the result of his upbringing) to listen, grow up and take responsibility for his actions.
Later, when Peter is ripped off by a shady wrestling promoter, he stands aside while the man is robbed, and during the getaway his uncle is killed
>>this is the climax that pushes Peter to the breaking point
It's all his fault. He had great power and great responsibility...and he dropped the ball. His actions lead to the death of the man he loved like a father. And it tears him to shreds.
Peter finally changes and takes action, telling Mary Jane he doesn’t love her (in order to protect her), and walks away. Into his destiny as Spiderman.