Biochemistry: Concepts and Connections (2nd Edition)
Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 2, Problem 9P

a. Suppose you wanted to make a buffer of exactly pH 7.00 using KH2PO4 and
Na2HPO4. If the final solution was 0.1 M in KH2PO4, what concentration of Na2HPO4 would you need?
b. Now assume you wish to make a buffer at the same pH, using the same substances, but want the total phosphate molarity ([HPO42- ]+[H2PO4-]) to equal 0.3. What concentrations of the KH2PO4 and Na2HPO4 would be required?

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