Hybridization probe - Wikipedia
In molecular biology, a hybridization probe (HP) is a fragment of DNA or RNA, usually 15–10000 nucleotides long, which can be radioactively or fluorescently labeled. HPs can be used to detect the presence of nucleotide sequences in analyzed RNA or DNA that are complementary to the sequence in the probe. [ 1 ]
Nucleic Acid Hybridization | Tools | Genetics - Biology Discussion
A hybridization probe is a nucleic-acid (DNA or RNA) fragment that is complementary to another nucleic-acid sequence and thus, when labeled (with radioisotope, fluorescent dye, etc.) can be used to identify complementary segments present in the nucleic-acid sequences of various microorganisms.
In Situ Hybridization (ISH) - National Center for Biotechnology Information
Nov 9, 2017 · In Situ Hybridization (ISH) is a technique that allows for precise localization of a specific segment of nucleic acid within a histologic section. The underlying basis of ISH is that nucleic acids, if preserved adequately within a histologic specimen, can be detected through the application of a complementary strand of nucleic acid to which a ...
Nucleic Acid Hybridization - an overview | ScienceDirect Topics
Nucleic acid hybridization involves bonding together short complementary nucleic acid strands (probes) to a target sequence. The probe is generally labeled with a radioisotope or fluorescent molecule, and the target sequence is typically bound to a nylon membrane or other solid surface.
DNA probes: applications of the principles of nucleic acid ...
Nucleic acid hybridization with a labeled probe is the only practical way to detect a complementary target sequence in a complex nucleic acid mixture. The first section of this article covers quantitative aspects of nucleic acid hybridization thermodynamics and kinetics.
Hybridization Probes – definition, uses, Preparation, Types.
Jun 3, 2024 · Hybridization probes are a small nucleotide sequence that is used for the detection of complementary sequences (DNA or RNA) in a nucleic acid sample. DNA Probes detects the complementary DNA with the help of a hybridization reaction.
Nucleic Acid Hybridization - Biology Ease
A hybridization probe is a nucleic-acid (DNA or RNA) fragment that is complementary to another nucleic-acid sequence and thus, when labeled (with radioisotope, fluorescent dye, etc) can be used to identify complementary segments present in the nucleic-acid sequences of various microorganisms.
Nucleic Acid Hybridizations - North Dakota State University
Probes are the primary tool used to identify complementary sequences of interest. Generally, the probe is a clone developed by inserting DNA into a vector. Most often these are plasmid clones. Probe - a single-stranded nucleic acid that has been radiolabelled and is used to identify a complimentary nucleic acid sequence that is membrane bound
Simulation-Guided DNA Probe Design for Consistently …
Here we show that simulations of the binding kinetics are both necessary and sufficient to design nucleic acid probe systems with consistently high specificity as they enable the discovery of an optimal combination of thermodynamic parameters.
Continuously Tunable Nucleic Acid Hybridization Probes
Here, we present a novel, on-the-fly method of tuning probe affinity and selectivity via the stoichiometry of auxiliary species, allowing independent and decoupled adjustment of hybridization yield for different probes in multiplexed assays.