Mitochondrial Ca2+ oscillation induces mitophagy initiation through …
Jun 19, 2021 · Our results provide a precise and noninvasive technology for research on mitophagy, which stimulates target mitochondria with little damage, and reveal mitoCa 2+ oscillation directly initiates...
CCCP-induced mitochondrial dysfunction - characterization and analysis ...
Mitochondrial dysfunction mediated by CCCP (carbonyl cyanide m-chlorophenyl hydrazone), an inhibitor of mitochondrial oxidative phosphorylation, evokes the integrated stress response (ISR), which is analyzed here by eIF2α phosphorylation and expression profiles of …
Current mechanistic insights into the CCCP-induced cell survival ...
Feb 1, 2018 · The mitophagy or autophagy response is induced and amplified by (i) the electrophilic properties of the lipophilic weak acids CCCP and FCCP; (ii) the production of ROS; and (iii) the increased concentration of cytosolic calcium following exposure to CCCP.
CCCP induces hepatic stellate cell activation and liver fibrogenesis ...
Nov 20, 2024 · We discovered that CCCP caused mitochondrial dysfunction in HSCs supported by increased mitochondrial ROS (mtROS) and depolarized mitochondrial membrane potential (MMP), reduced mitochondrial DNA (mtDNA) with ATP depletion.
Current mechanistic insights into the CCCP-induced cell ... - PubMed
CCCP and FCCP are now being increasingly used for investigating the mechanisms of autophagy by inducing mitochondrial degradation through the disruption of the mitochondrial membrane potential (ΔΨm).
PGAM5 regulates PINK1/Parkin-mediated mitophagy via DRP1 in CCCP …
Mar 1, 2018 · We found that CCCP decreased mitochondrial membrane potential, resulting in mitochondrial dysfunction. CCCP increased PGAM5, dynamin-related protein 1 (DRP1), and optic atrophy 1 (OPA1) expression of the mitochondrial fraction in a time-dependent manner.
Current mechanistic insights into the CCCP-induced cell survival ...
Feb 1, 2018 · CCCP and FCCP are now being increasingly used for investigating the mechanisms of autophagy by inducing mitochondrial degradation through the disruption of the mitochondrial membrane potential (ΔΨm).
The pharmacological regulation of cellular mitophagy
Jan 19, 2017 · Current approaches to initiate mitophagy include acute dissipation of the mitochondrial membrane potential (ΔΨ m) by mitochondrial uncouplers (for example, FCCP/CCCP) and the use of antimycin A...
LC3-Mediated Mitophagy After CCCP or Vibrio splendidus …
May 20, 2022 · Mitochondrial selective autophagy, known as mitophagy, surveils the mitochondrial population by eliminating superfluous and/or impaired organelles to mediate cellular survival and viability in response to injury/trauma and infection.
Full article: The protonophore CCCP interferes with lysosomal ...
Sep 27, 2013 · We showed that CCCP interfered with lysosomal function and autophagosomal degradation in both yeast and mammalian cells, inhibited starvation-induced mitophagy in mammalian cells, and blocked the induction of mitophagy in yeast cells.
- Some results have been removed