BV2 Cells: Key Microglial Models in Neuroinflammation
Apr 29, 2025 · Explore the characteristics, activation methods, and interactions of BV2 cells as a microglial model to study neuroinflammation and cellular responses. Microglial cells are the …
The suitability of BV2 cells as alternative model system for primary ...
Here, we re-evaluated strengths and potential short-comings of BV-2 cells. Their response to lipopolysaccharide was compared with the response of microglia in vitro and in vivo.
An Overview of in vitro Methods to Study Microglia - PMC
The BV2 cell line was generated by transduction of neonatal primary microglia with the v-raf/v-myc carrying J2 retrovirus. Immortalized, transformed cells survived for more than 4 weeks in …
In vitro examination of microglia-neuron crosstalk with BV2 cells, …
Our data revealed that LPS-treated BV2 cells and primary cultures of microglia could release IL-1β into culture medium that had a stimulatory effect on primary cultures of hypothalamic …
Deep proteomic analysis of microglia reveals fundamental …
Nov 26, 2024 · Our database includes the BV2 cell line proteome, a commonly used in vitro model to analyze microglia-like responses. The BV2 cell line is derived from primary mouse …
The effects of insulin on the inflammatory activity of BV2 microglia …
We now show that in BV2 microglia, thr308 is phosphorylated at 2 hours after insulin and LPS administration, more so than ser473. LPS led to an increase in ser473 phosphorylation more …
Cellosaurus cell line BV-2 (CVCL_0182)
Genome-wide inspection of chromosomal aberrations in microglia BV-2 cells by array-based comparative genomic hybridization. The suitability of BV2 cells as alternative model system for …
OTUD1 positively regulates microglia neuroinflammation and
May 7, 2025 · We also examined the protein levels of OTUD1 in a cultured microglia BV2 cell line. OTUD1 levels were increased in time- and dose-dependent manners in BV2 cells after Aβ …
Morphology, Growth, and Troubleshooting in BV2 Cell Culture
Dec 31, 2024 · The BV2 cell line was established by E. Blasi in 1990 through the immortalization of mouse microglia cells, achieved by retroviral-mediated transfection with v-raf/v-myc. BV2 …
In vitro examination of microglia-neuron crosstalk with BV2 …
Aug 22, 2018 · In this study, we used BV2 cells and primary cultures of glia from neonatal rats to explore the interaction between microglia and hypothalamic neurons in vitro. …