A major bottleneck in curing HIV (human immunodeficiency virus) is that the virus can hide in an inactive form within resting ...

RNA transfection for direct functional analysis of RNA species is becoming increasingly popular. This is due, in part, to exploding interest in RNA interference (RNAi), a gene-silencing technique used ...

20 Gene silencing: Transfection of cells with RNA interference (RNAi) molecules such as small interfering RNA (siRNA), which disintegrate the mRNA, or micro-RNA (miRNA), which suppress the translation ...

We analyzed the efficiency of tumor RNA transfection into DC using different sources of RNA as well as delivery strategies including electroporation, lipofection and CD71-receptor-based delivery.

RNA just needs to get into the cytoplasm, eliminating a step in the transfection process,” said Xavier de Mollerat du Jeu, Ph.D., senior staff scientist, molecular biology, Life Technologies.

The company offers two sets of transfection reagents, one for DNA and another for RNA. The DNA reagent is based on cationic polymer technology from MIT. Stemgent offers three DNA reagents ...

Transfection of small RNAs (such as small interfering ... the design of siRNA and short hairpin RNA (shRNA) genomic screens and siRNA therapeutics. Figure 1: Schematic of the hypothesis that ...

If you work in a lab, whether you are researching the cutting edge of cell and gene therapy development or running a simple molecular binding screen, the chances are you have done some transfection in ...

Simply put, transfection is a foundational technique by which foreign nucleic acids such as DNA or RNA, can be introduced into mammalian host cells through non-viral means (e.g., liposomes, calcium ...

Transfection is a crucial non-viral technique for introducing DNA or RNA into cells, yet achieving high efficiency and consistency can be challenging, particularly with difficult cell types.

Caption Researchers use modified RNA transfection to correct genetic dysfunction in heart stem cells derived from Barth syndrome patients. The series of images show how inserting modified RNA into ...