A variety of procedures can be used to remove these contaminants, leaving the DNA in a pure form. The standard way to de-proteinize a cell extract is to add phenol or a 1:1 mixture of ...
Set up two reactions for digestion of genomic DNA: Incubate the two reactions at 37 °C for 3 h. Extract the reactions with phenol/chloroform (1:1, v/v), recover the DNA by precipitation with ...
To purify the amplification products, extract each reaction once with phenol/chloroform/isoamyl alcohol (25: 24:1, v/v/v) and then with chloroform/isoamyl alcohol (29:1, v/v) and recover the DNA ...
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