You were given a mixture of two proteins with different isoelectric points and molecular weights:• Protein X: pI 4.2, MW 42,000• Protein Y: pI 9.8, MW 90,000Using a Tris-glycine discontinuous native gel (pH8.3) electrophoresis system with a running gel of 12%, only a single band was observed upon protein staining after electrophoresis. Explain the observed result and discuss possible factors affecting protein migration in this system.
You were given a mixture of two proteins with different isoelectric points and molecular weights:• Protein X: pI 4.2, MW 42,000• Protein Y: pI 9.8, MW 90,000Using a Tris-glycine discontinuous native gel (pH8.3) electrophoresis system with a running gel of 12%, only a single band was observed upon protein staining after electrophoresis. Explain the observed result and discuss possible factors affecting protein migration in this system.
Biochemistry
9th Edition
ISBN:9781305961135
Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Chapter3: Amino Acids And Peptides
Section: Chapter Questions
Problem 10RE: MATHEMATICAL Calculate the isoelectric point of each of the following amino acids: glutamic acid,...
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Question
You were given a mixture of two proteins with different isoelectric points and molecular weights:
• Protein X: pI 4.2, MW 42,000
• Protein Y: pI 9.8, MW 90,000
Using a Tris-glycine discontinuous native gel (pH8.3) electrophoresis system with a running gel
of 12%, only a single band was observed upon protein staining after electrophoresis. Explain the
observed result and discuss possible factors affecting protein migration in this system.
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