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Consider a chromatography column in which Vs= Vm/5. Find the retention factor if Kd= 3 and Kd= 30.
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- Consider a 22.0 cm long column with a plate height of 0.0125 mm, a flow rate of 1.0 mL/min, and a Vm =1.2 mL (peak shapes are all symmetric). What is the solute retention time, retention volume, and peak width at half height when k’ = 7.2?In molecular exclusion (size exclusion) chromatography, the molecules have the longest retention times. a) the larger molecules b) the smaller moleculesA solvent passes through a chromatography column in 3.87 min, but the solute requires 7.60 min. What is the retention factor, k? k = What fraction of the time does the solute spend in the mobile phase in the column? t fraction in mobile phase = The volume of the stationary phase is 0.133 times the volume of the mobile phase in the column (Vs = 0.133VM). What is the distribution constant, Kp, for this system? KD = %3D
- Internal standard. A mixture containing 12.8 mM analyte (X) and 44.4 mM standard (S) gave chromatographic peak areas of 306 for X and 511 for S. A second solution containing an unknown quantity of X plus 55.5 mM S had peak areas of 251 for X and 563 for S. Find [X] in the second solution.5All types of chromatography depend on partitioning a sample between a stationary phase and a mobile phase. Identify the stationary phase and mobile phase for each of the following types of chromatography. -TLC -GC
- The options are larger or smaller.Two components of a 12-mg sample are adequately separated by chromatography through a column 1.5 cm in diameter and 25 cm long at a flow rate of 0.8 mL/min. What size column and what flow rate should be used to obtain similar separation with a 250-mg sample?Describe 4 similarities and 4 differences between Thin layer chromatography (TLC) and column chromatography.
- 4.Consider the peaks for pentafluorobenzene and benzene in the gas chromatogram shown here. The elution time for unretained solute is 1.06 min. The open tubular column is 30.0 m in length and 0.530 mm in diameter, with a layer of stationary phase 3.0 μm thick on the inner wall. a) Measruing the width, w, at the baseline on the chromatogram, find the number of plates for these two compounds b) Use your answer to (a) to find the resolution between the two peaks c) Using the number of plates N=sqrtN1*N2 with the values from (a) calcuate what the resolution should be and compare your answer with the measured resolution in bConsider a separation performed on a 30.0 mm long open tubular column with a 0.50 mm diameter and a 2.0 µm thick stationary phase. Compound A eluted at 12.63 min, compound B eluted at 13.24 min, and the unretained solvent eluted at 1.205 min. What are the adjusted retention times, t, and retention factors, k, for compounds A and B? TRA KA = || TRB = KB = = min minYou are trying to separate a sample with 2 components: X and Y with GC. X has a lower boiling point than Y. Which one has a higher retention time? What is the most common way to increase retention of analytes in gas chromatography?
