A chromatogram with ideal Gaussian bands has tR = 9.0 minutes and w1/2 = 2.0 minutes. Find the number of theoretical plates that are present, and calculate the height of each theoretical plate if the column is 10 centimeters long.
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A chromatogram with ideal Gaussian bands has tR = 9.0 minutes and w1/2 = 2.0 minutes. Find the number of theoretical plates that are present, and calculate the height of each theoretical plate if the column is 10 centimeters long.
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- One method for the quantitative determination of the concentration of constituents in a sample analyzed by gas chromatography is area normalization. Here, complete elution of all the sample constituents is necessary. The area of each peak is then measured and corrected for differences in detector response to the different eluates. This correction involves dividing the area by an empirically determined correction factor. The concentration of the analyte is found from the ratio of its corrected area to the total corrected area of all peaks. For a chromatogram containing three peaks, the relative areas were found to be 16.4, 45.2 and 30.2, in order of increasing retention time. Calculate the percentage of each compound if the relative detector responses were 0.60, 0.78 and 0.88, respectively.A gel filtration column has a radius (r) of 0.80 cm and a length (l) of 20.0 cm. Calculate the total volume of the column, which is equal to pr2l.The options are larger or smaller.
- What are the advantages and disadvantages of using external standards in instrumental analysis?Which type of chromatography is most useful for determining the concentrations of minerals in bottled water? size exclusion ion-exchange gas thin layer adsorptionAs part of an analytical chemistry laboratory course, a student measured the Ca2+ content in two water samples, city-supplied drinking water and well-supplied drinking water, using two different analytical methods, flame atomic absorption spectrometry (FAAS) and EDTA complexometric titration. The results of this experiment are given in the table as the mean Ca2+concentration (?¯) and standard deviation (?) in parts per million (ppm). Each sample was measured five times (n=5) by each method. Method City-Supplied Drinking Water (?¯±?x¯±s) Well-Supplied Drinking Water (?¯±?x¯±s) FAAS 57.57±0.68 ppm 64.77±0.70 ppm EDTA titration 58.32±0.96 ppm 65.62±0.97 ppm Method Comparison: For each drinking water sample (city and well), compare the Ca2+ content measured by FAAS and EDTA titration. Calculate the ? value for each sample. Do the methods produce statistically different results at the 95% confidence level when measuring the Ca2+content of the city-supplied drinking water? Do the…
- An atomic absorption method for the determination of the amount of iron present in used jet engine oil was found from pooling 30 triplicate analyses to have a standard deviation s = 3.6 mg Fe/mL. If s is a good estimate of σ, calculate the 95 and 99% confidence intervals for the result 18.5 mg Fe/mL if it was based on (a) a single analysis, (b) the mean of two analyses, and (c) the mean of four analyses.You have obtained a gas chromatogram of a two component mixture of compounds A and B. The height of peak A is 3.84 cm. and the width at half height is 1 cm. The height of peak B is 3.49 cm. and the width at half height is 1.36 cm. The percentage of a component in the mixture is proportional to peak area. Estimate peak areas by multiplying height by width at half height. What was the percentage of compound B in the mixture? (Enter your answer as a number without any percent symbol or the word percent.)Chromatography involves the separation of two or more compounds/ions caused by their molecular interactions with two phases. True False
- what is chromatography? Explain different types of chromatography and explain how to seperate compounds. indicate stationary phase mobile phase.Many ions form colored solutions when dissolved in water. For example, aqueous Cu2+ solutions are light blue and aqueous Ni2+ solutions look light green. These metals in particularare often found in stainless steel. Would you expect their presence in your samples to interfere in the spectrophotometric analysis of permanganate at 525 nm? Why or why not? I think the answer is that I would expect the Cu2+ in my samples to interfere but not Ni2+ because the Ni2+ is green, signifying a higher wave length that the spectrometer wouldn't be able to pick up on because it was only set to 525 (blue-ish) where as the green is 530-ish. I just want to make sure thanks!The answer is 0.372 ug/mL
